Trifluoroacetic Acid
Trifluoroacetic acid is a corrosive organofluorine compound that is structurally analogous to, but stronger than, acetic acid. Available in various quantities and reagent grades, it is used in NMR spectroscopy, mass spectrometry, organic synthesis, etc.
Almost 100,000-fold more acidic than acetic acid, TFA is widely used in organic chemistry. TFA is used as a reagent in organic synthesis because of its properties: volatility, organic solvent solubility, and acidic strength. It is less oxidizing than sulfuric acid and is more readily available in its anhydrous form than some other acids . Other features include:
- Used in acid-catalyzed reactions, especially peptide synthesis (to cleave esters)
- Dissolves protein when mixed with liquid SO2
- Removes t-butyl derived side-chain protecting groups in Fmoc peptide synthesis
- Can remove the t-butoxycarbonyl protecting group in organic synthesis
- At low concentrations, acts as an ion-pairing agent for peptides and small proteins in organic compound liquid chromatography
- For acid-stable materials, can be a solvent for NMR spectroscopy
- Acts as a calibrant in mass spectrometry
- Used to produce trifluoroacetate salts
- An ingredient in adhesives, sealants, paints, and coatings
When TFA combines with bases and metals, especially light metals, a strong exothermic reaction occurs. When mixed with lithium aluminum hydride (LAH), the reaction is explosive.
Although nonflammable, TFA is corrosive to skin, eyes, and mucous membranes and requires careful use and handling. It is harmful when inhaled, causes severe skin burns and eye damage, and is toxic to aquatic organisms at even low concentrations.
TFA is also a product of the metabolic breakdown of the anesthetic agent halothane. It is the suspected cause of halothane-induced hepatitis.
Chem-Impex International, Inc. Trifluoroacetic acid | 76-05-1 | MFCD00004169 | 100G
Trifluoroacetic acid, 76-05-1, MFCD00004169, 100G
Chem-Impex International, Inc. Trifluoroacetic acid | 76-05-1 | MFCD00004169 | 250G
Trifluoroacetic acid, 76-05-1, MFCD00004169, 250G
Cambridge Isotope Laboratories Trifluoroacetic Acid-D (D, 99.5%), 10 G Amp, 599-00-8
This item has a minimum order quantity of 2 per supplier requirements
Trifluoroacetic Acid-D (D, 99.5%), 10 G Amp, 599-00-8
Supply Solutions Trifluoroacetic acid | 76-05-1 | 114.02 g/mol | 10x1ml
Trifluoroacetic acid | 76-05-1 | 114.02 g/mol | 10x1ml
Sigma Aldrich Fine Chemicals Biosciences Trifluoroacetic anhydride ReagentPlus(R), >=99% | 407-25-0 | MFCD00000416 | 25KG
Trifluoroacetic anhydride ReagentPlus(R), >=99% | Purity: >=99% | Mol Wt: 210.03 | 407-25-0 | MFCD00000416 | 25KG
Sigma Aldrich Fine Chemicals Biosciences Trifluoroacetic anhydride ReagentPlus(R), >=99% | 407-25-0 | MFCD00000416 | 4X100G
Trifluoroacetic anhydride ReagentPlus(R), >=99% | Purity: >=99% | Mol Wt: 210.03 | 407-25-0 | MFCD00000416 | 4X100G
Sigma Aldrich Fine Chemicals Biosciences Trifluoroacetic acid ReagentPlus, 99, 76-05-1, MFCD00004169
Trifluoroacetic acid ReagentPlus, 99
Apexbio Technology LLC 2X HyperFusion High-Fidelity Master Mix 10X1ml
2X HyperFusion High-Fidelity Master Mix provides high fidelity strong amplification capability and faster amplification speed for most PCR applications HyperFusion DNA polymerase is composed of a Pyrococcus-like proofreading polymerase fused with a DNA-binding domain Its unique structure which combines a novel Pyrococcus-like enzyme and a domain enhancing synthesis capability increases fidelity and speed Due to its high fidelity HyperFusion DNA polymerase is an ideal choice for molecular cloning or other downstream applications HyperFusion DNA polymerase is among the DNA polymerases with the highest fidelity having a 50-fold lower error rate compared to Taq DNA polymerase and a 6-fold lower error rate than Pyrococcus Furious DNA polymerase HyperFusion DNA polymerase exhibits 5 to 3 polymerase activity and 3 to 5 exonuclease activity It generates blunt-ended PCR products without A-overhangs The extension rate is approximately 15-30 sec per kb (up to 10 kb in length)
Sigma Aldrich Fine Chemicals Biosciences Trifluoroacetic acid 99 fo100G
TRIFLUOROACETIC Trifluoroacetic acid 99 fo100G
Sigma Aldrich Fine Chemicals Biosciences Trifluoroacetic acid Reage
TRIFLUOROACETIC Trifluoroacetic acid Reage
Apexbio Technology LLC Pyrophosphatase Inorganic (E. coli) 10 units
Pyrophosphatase Inorganic (E coli) purified from recombinant E coli strain carrying the E coli inorganic pyrophosphatase gene Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme that catalyzes the hydrolysis of inorganic pyrophosphate into orthophosphate
Apexbio Technology LLC Protease Inhibitor Cocktail for bacterial cell extracts (100 X in DMSO) 10x(1ml A 1ml B)
Under stable environmental conditions endogenous protein synthesis and degradation are balanced thus maintaining stable cellular protein levels Cell crude extracts contain numerous endogenous enzymes such as phosphatases and proteases that can degrade proteins in the extracts During in vitro protein extraction from cells and tissues protein synthesis practically ceases while protein degradation increases The optimal way to enhance yields of intact proteins is to add inhibitors for these known enzymes The Protease Inhibitor Cocktail for bacterial cell extracts (100X in DMSO) is designed for bacterial extracts to enhance the stability of proteins This cocktail inhibits proteases responsible for degrading non-phosphorylated or phosphorylated protein substrates This Protease Inhibitor Cocktail is provided as a ready-to-use solution and it is formulated in DMSO
Apexbio Technology LLC 2X hyperfusion plus master mix 10X1ml
Our 2X Hyperfusion Plus Master Mix (With dye) product offers high fidelity strong amplification performance and faster extension speed simultaneously making it suitable for most PCR applications Hyperfusion Plus DNA Polymerase is created by fusing a Pyrococcus-like proofreading polymerase with a DNA binding domain providing increased fidelity and speed Hyperfusion Plus DNA Polymerase owns high fidelity 50 times lower error rate than Taq DNA Polymerase and 6 times lower error compared to Pyrococcus Furious DNA Polymerase Hyperfusion Plus DNA Polymerase exhibits both 5 to 3 polymerase activity and 3 to 5 exonuclease (proofreading) activity producing blunt-ended amplification products rather than A-tailed ends (commonly produced by Taq polymerase) Experimental results demonstrate that our Hyperfusion Plus polymerase can amplify genome fragments as long as 20 1 kb